Evaluation of ultraviolet photodissociation tandem mass spectrometry for the structural assignment of unsaturated fatty acid double bond positional isomers

M Fang; Y Rustam; M Palmieri; OM Sieber; GE Reid

Journal article

Evaluation of ultraviolet photodissociation tandem mass spectrometry for the structural assignment of unsaturated fatty acid double bond positional isomers

M Fang, Y Rustam, M Palmieri, OM Sieber, GE Reid

Analytical and Bioanalytical Chemistry | SPRINGER HEIDELBERG | Published : 2020

DOI: 10.1007/s00216-020-02446-6

Abstract

Fatty acids are a major source of structural diversity within the lipidome due to variations in their acyl chain lengths, branching, and cyclization, as well as the number, position, and stereochemistry of double bonds within their mono- and poly-unsaturated species. Here, the utility of 193 nm UltraViolet PhotoDissociation tandem mass spectrometry (UVPD-MS/MS) has been evaluated for the detailed structural characterization of a series of unsaturated fatty acid lipid species. UVPD-MS/MS of unsaturated fatty acids is shown to yield pairs of unique diagnostic product ions resulting from cleavages adjacent to their C=C double bonds, enabling unambiguous localization of the site(s) of unsaturati..

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University of Melbourne Researchers

Michelle Palmieri Author

Oliver Sieber Author

Gavin Reid Author

Related Projects (1)

Cutting the Fat: Photodissociation Mass Spectrometry for Lipidome Analysis

This project aims to develop and apply novel bioanalytical mass spectrometry-based methods and workflows to illuminate the otherwise hidden ..

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This research was supported by funding from the Australian Research Council (Grant DP190102464) to GER and OMS, and from the National Health and Medical Research Council (Grant APP1156778) to OMS and GER. OMS is a National Health and Medical Research Council Senior Research Fellow (Grant APP1136119).